DOI: 10.1002/bip.360030301. Kinetics and equilibria in the acid denaturation of deoxyribonucleic acids from various sources

Kinetics and equilibria in the acid denaturation of deoxyribonucleic acids from various sources

10.1002/bip.360030301

Crossref journal-article

Wiley

Biopolymers (311)

Abstract

AbstractThe acid denaturation of a variety of DNA‡ samples with guanine–cytosine (GC) contents ranging from 37–64% has been investigated by means of potentiometric, spectrophotometric, and thermal titrations, including spectrophotometric titrations in a stopped‐flow fast reaction apparatus. As the pH of a solution of native DNA is lowered from the neutral range the net charge on the molecule becomes more positive as a result of the addition of protons to the bases adenine, cytosine, and to a lesser extent guanine. At a fairly sharply defined value of the charge a conformational transition sets in which is completed after the charge has increased in the positive direction by an additional 0.4–0.5 unit per base pair. The threshold value of the charge is dependent on the composition of the DNA as well as various experimental parameters such as temperature and ionic strength. At 25°C. in 0.1M NaCl the threshold value is approximately 0.55 charge unit per base pair relative to pH 6 for a GC content of 37% and 0.78 for 64% CG. The conformational transition is accompanied by heat absorption amounting, in the case of salmon DNA in 0.1M NaCl, to 3020 ± 150, 4920 ± 200, and 5700 ± 250 cal./mole of base pairs at 5, 25, and 45°C., respectively. If it is assumed that the heats of ionization of the bases are the same in denatured DNA as in the isolated nucleotides, the result at 25° C. can be corrected by using previously determined heats of ionization to give for the hypothetical process, DNA (native, pH 6) → DNA (denatured, pH 6) ΔH = 8300 cal./mole of base pairs. ΔH appears to be independent of DNA composition, an average value of 8000 cal./mole of base pairs being obtained for DNA samples having GC contents ranging from 37–64%. The calorimetric results are consistent with the view that hydrophobic interactions are important in the stabilization of the DNA double helix. The kinetics of the acid denaturation of salmon DNA is complex.

Bibliography

Bunville, L. G., Geiduschek, E. P., Rawitscher, M. A., & Sturtevant, J. M. (1965). Kinetics and equilibria in the acid denaturation of deoxyribonucleic acids from various sources. Biopolymers, 3(3), 213â240. Portico.

Authors 4

L. G. Bunville (first)
E. P. Geiduschek (additional)
M. A. Rawitscher (additional)
J. M. Sturtevant (additional)

References 44 Referenced 75

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Dates

Type	When
Created	20 years, 7 months ago (Dec. 30, 2004, 10:22 p.m.)
Deposited	1 year, 9 months ago (Nov. 12, 2023, 8:35 a.m.)
Indexed	1 year, 6 months ago (Feb. 8, 2024, 9:07 p.m.)
Issued	60 years, 2 months ago (June 1, 1965)
Published	60 years, 2 months ago (June 1, 1965)
Published Online	21 years, 6 months ago (Feb. 1, 2004)
Published Print	60 years, 2 months ago (June 1, 1965)

Funders 0

None

BibTeX

@article{Bunville_1965, title={Kinetics and equilibria in the acid denaturation of deoxyribonucleic acids from various sources}, volume={3}, ISSN={1097-0282}, url={http://dx.doi.org/10.1002/bip.360030301}, DOI={10.1002/bip.360030301}, number={3}, journal={Biopolymers}, publisher={Wiley}, author={Bunville, L. G. and Geiduschek, E. P. and Rawitscher, M. A. and Sturtevant, J. M.}, year={1965}, month=jun, pages={213–240} }

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