DOI: 10.1002/bip.1975.360140802. Adsorption behaviour of globular proteins at the water/mercury interface

Adsorption behaviour of globular proteins at the water/mercury interface

10.1002/bip.1975.360140802

Crossref journal-article

Wiley

Biopolymers (311)

Abstract

AbstractThe adsorption of globular proteins at solid/liquid or liquid/liquid interfaces provides evidence of unfolded molecular conformation. Proteins with high apolar character are strongly unfolded, while those with high polar character are generally incompletely unfolded. Structural changes of globular proteins at adsorption on mercury electrodes were studied by ac polarography and capacity–time curves. The surface area per molecule of nine globular proteins was determined from the adsorption kinetics at the dropping mercury electrode. For all the proteins investigated, this value was greater than the maximal molecular cross section of the native proteins. The surface area was about 19 Å2 per amino acid residue, which coincides with the value for unfolded proteins at the water/air interface. Differences between dropping mercury electrode and hanging drop mercury electrode occurred only with lysozyme and phosphorylase; for the other proteins, the structure of the adsorption layer was independent of the time of interaction at the electrode. Since not all of the reducible groups of the adsorbed proteins come into contact with the electrode, the flattening should be incomplete.

Bibliography

Scheller, F., JÃ¤nchen, M., & PrÃ¼mke, H. (1975). Adsorption behaviour of globular proteins at the water/mercury interface. Biopolymers, 14(8), 1553â1563. Portico.

Authors 3

Frieder Scheller (first)
Michael Jänchen (additional)
Hans‐Jörg Prümke (additional)

References 50 Referenced 65

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Dates

Type	When
Created	20 years, 8 months ago (Dec. 29, 2004, 7:40 p.m.)
Deposited	1 year, 9 months ago (Nov. 12, 2023, 5:15 a.m.)
Indexed	1 year, 9 months ago (Nov. 15, 2023, 2:22 p.m.)
Issued	50 years, 1 month ago (Aug. 1, 1975)
Published	50 years, 1 month ago (Aug. 1, 1975)
Published Online	21 years, 7 months ago (Feb. 1, 2004)
Published Print	50 years, 1 month ago (Aug. 1, 1975)

Funders 0

None

BibTeX

@article{Scheller_1975, title={Adsorption behaviour of globular proteins at the water/mercury interface}, volume={14}, ISSN={1097-0282}, url={http://dx.doi.org/10.1002/bip.1975.360140802}, DOI={10.1002/bip.1975.360140802}, number={8}, journal={Biopolymers}, publisher={Wiley}, author={Scheller, Frieder and Jänchen, Michael and Prümke, Hans‐Jörg}, year={1975}, month=aug, pages={1553–1563} }

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