DOI: 10.1002/bip.1970.360090310. Simplified quantitative electron microscopy of biopolymers

Simplified quantitative electron microscopy of biopolymers

10.1002/bip.1970.360090310

Crossref journal-article

Wiley

Biopolymers (311)

Abstract

AbstractShape and molecular weight of DNA can be obtained with a new method involving spontaneous surface adsorption of DNA or of other macromolecules from a solution which contains formaldehyde and a critical amount of cytochrome c. Subsequent transfer to specimen grids and electron microscopy shows individual molecules suitable for quantitative evaluation. Adsorption of DNA and fd bacteriophages is proportional to (time)0.75. Contour lengths of double‐stranded DNA are not influenced by formaldehyde. A modification of this method requires only 2 × 10−5 μg DNA.

Bibliography

Lang, D., & Mitani, M. (1970). Simplified quantitative electron microscopy of biopolymers. Biopolymers, 9(3), 373â379. Portico.

Authors 2

D. Lang (first)
Michiko Mitani (additional)

References 9 Referenced 216

10.1515/znb-1959-1206
{'key': 'e_1_2_1_3_2', 'first-page': '142', 'volume': '88', 'author': 'Lang D.', 'year': '1964', 'journal-title': 'Biochim. Biophys. Acta'} / Biochim. Biophys. Acta by Lang D. (1964)
10.1016/S0022-2836(67)80024-X
10.1016/0022-2836(68)90225-8
10.1126/science.161.3847.1246
T.Nisioka M.Mitani andR.Clowes J. Bacteriol. in press.
10.1016/0022-2836(69)90069-2
10.1073/pnas.49.5.748
10.1016/S0022-2836(68)80012-9

Dates

Type	When
Created	20 years, 7 months ago (Dec. 29, 2004, 5:15 a.m.)
Deposited	1 year, 9 months ago (Nov. 11, 2023, 10:50 p.m.)
Indexed	1 year, 6 months ago (Jan. 27, 2024, 4:21 a.m.)
Issued	55 years, 5 months ago (March 1, 1970)
Published	55 years, 5 months ago (March 1, 1970)
Published Online	21 years, 6 months ago (Feb. 1, 2004)
Published Print	55 years, 5 months ago (March 1, 1970)

Funders 0

None

BibTeX

@article{Lang_1970, title={Simplified quantitative electron microscopy of biopolymers}, volume={9}, ISSN={1097-0282}, url={http://dx.doi.org/10.1002/bip.1970.360090310}, DOI={10.1002/bip.1970.360090310}, number={3}, journal={Biopolymers}, publisher={Wiley}, author={Lang, D. and Mitani, Michiko}, year={1970}, month=mar, pages={373–379} }

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