DOI: 10.1002/bip.1969.360070408. Determination of DNA base compositions from melting profiles in dilute buffers

Determination of DNA base compositions from melting profiles in dilute buffers

10.1002/bip.1969.360070408

Crossref journal-article

Wiley

Biopolymers (311)

Abstract

AbstractEquations were determined for the dependency of the melting temperature (Tm) of DNA upon the logarithm of the sodium ion concentration, for four DNA samples of widely different base compositions (θGC). The slopes of these Tm versus log M equations wore found to decrease with increasing θG Cof the samples. An empirical equation relating Tm, log M (Na+) and θG C was derived, which also accounts for differences in Tm versus log M slopes. Data from the literature for some synthetic polynucleotides and for the crab(Cancer pagarus) satellite poly AT are discussed in relation to the above finding. The changes in Tm versus log M slopes with θG C are interpreted in terms of changes in the thermodynamic parameters ΔS and ΔH with base composition.

Bibliography

Owen, R. J., Hill, L. R., & Lapage, S. P. (1969). Determination of DNA base compositions from melting profiles in dilute buffers. Biopolymers, 7(4), 503â516. Portico.

Authors 3

R. J. Owen (first)
L. R. Hill (additional)
S. P. Lapage (additional)

References 31 Referenced 228

10.1002/bip.360030207
10.1016/S0022-2836(62)80066-7
10.1099/00221287-33-2-243
10.1016/S0022-2836(62)80119-3
10.1128/jb.90.1.136-140.1965 / J. Bacteriol by Silvestri L. G. (1965)
10.1099/00221287-38-2-243
10.1016/S0022-2836(62)80082-5
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10.1016/S0022-2836(61)80047-8
{'key': 'e_1_2_1_15_2', 'first-page': '177', 'volume-title': 'Identification Methods for Microbiologists, Part B', 'author': 'Hill L. R.', 'year': '1968'} / Identification Methods for Microbiologists, Part B by Hill L. R. (1968)
{'key': 'e_1_2_1_16_2', 'first-page': '1', 'volume': '12', 'author': 'Doty P.', 'year': '1959', 'journal-title': 'Erookhaven Symp. Biol.'} / Erookhaven Symp. Biol. by Doty P. (1959)
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10.1038/198588a0
{'key': 'e_1_2_1_23_2', 'first-page': '1446', 'volume': '24', 'author': 'Chambelin M. J.', 'year': '1965', 'journal-title': 'Fed. Proc.'} / Fed. Proc. by Chambelin M. J. (1965)
10.1099/00221287-46-3-369
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10.1002/bip.1967.360051010
{'key': 'e_1_2_1_31_2', 'first-page': '481', 'volume': '11', 'author': 'Barszez D.', 'year': '1964', 'journal-title': 'Acta Biochem. Polon.'} / Acta Biochem. Polon. by Barszez D. (1964)

Dates

Type	When
Created	20 years, 3 months ago (May 27, 2005, 11:37 a.m.)
Deposited	1 year, 9 months ago (Nov. 12, 2023, 9:37 a.m.)
Indexed	4 weeks, 1 day ago (Aug. 7, 2025, 4:50 p.m.)
Issued	56 years, 5 months ago (April 1, 1969)
Published	56 years, 5 months ago (April 1, 1969)
Published Online	21 years, 7 months ago (Feb. 1, 2004)
Published Print	56 years, 5 months ago (April 1, 1969)

Funders 0

None

BibTeX

@article{Owen_1969, title={Determination of DNA base compositions from melting profiles in dilute buffers}, volume={7}, ISSN={1097-0282}, url={http://dx.doi.org/10.1002/bip.1969.360070408}, DOI={10.1002/bip.1969.360070408}, number={4}, journal={Biopolymers}, publisher={Wiley}, author={Owen, R. J. and Hill, L. R. and Lapage, S. P.}, year={1969}, month=apr, pages={503–516} }

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