DOI: 10.1002/bip.1969.360070309. Hydrogen‐tritium exchange of the random chain polypeptide

Hydrogen‐tritium exchange of the random chain polypeptide

10.1002/bip.1969.360070309

Crossref journal-article

Wiley

Biopolymers (311)

Abstract

AbstractThe Hydrogen–tritium exchange character of poly‐D,L‐alanine was studied in detail as a model for the hydrogen exchange behavior of the unhindered, polymeric peptide group. The random chain nature of poly‐D,L‐alanine was evident in the uniformity of exchange rate of all its hydrogens and in the similarity between this rate and that of random chain poly‐D,L‐lysine and other known, unhindered secondary amide groups. An equilibrium isotope effect favoring the binding of tritium over protium to the extent of 21% was measured. Specific acid and base catalysis of the exchange and the absence of detectable general catalysis were demonstrated. Apparent energy of activation is 17 kcal/mole for deprotonation, largely due to dependence of Kw on temperature, and 15 kcal/mole for protonation, which correlates with the extreme apparent pK. The hydrogen –tritium exchange half‐time rate; of poly‐D,L‐alamine at any pH and temperature (T: °C) is given by the equation:

Bibliography

Englander, S. W., & Poulsen, A. (1969). Hydrogenâtritium exchange of the random chain polypeptide. Biopolymers, 7(3), 379â393. Portico.

Authors 2

S. W. Englander (first)
A. Poulsen (additional)

References 22 Referenced 119

10.1016/0003-9861(57)90478-2
10.1016/0022-2836(67)90215-X
10.1016/0006-3002(60)90843-X
10.1021/bi00906a026
10.1021/bi00904a030
10.1126/science.138.3542.830
10.1021/ja01090a033
{'key': 'e_1_2_1_8_2', 'first-page': '325', 'volume': '242', 'author': 'Downie A. R.', 'year': '1967', 'journal-title': 'Proc. Roy. Soc., (London)'} / Proc. Roy. Soc., (London) by Downie A. R. (1967)
{'key': 'e_1_2_1_9_2', 'volume-title': 'Polyamino Acids, Polypeptides and Proteins', 'author': 'Elliott A.', 'year': '1962'} / Polyamino Acids, Polypeptides and Proteins by Elliott A. (1962)
10.1016/0006-3002(60)90842-8
10.1021/ja00891a035
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10.1002/anie.196400011
{'key': 'e_1_2_1_16_2', 'first-page': '255', 'volume-title': 'Fast Reactions and Primary Processes in Chemical Kinetics', 'author': 'Eyring E. M.', 'year': '1968'} / Fast Reactions and Primary Processes in Chemical Kinetics by Eyring E. M. (1968)
10.1002/bip.1965.360030506
10.1016/S0065-3233(08)60129-1
10.1021/ja01510a014
10.1016/0006-3002(60)90090-1
S. W.EnglanderandR.Stayle in preparation.
{'key': 'e_1_2_1_22_2', 'first-page': '346', 'volume-title': 'Poly‐α‐Amino Acids', 'author': 'Englander S. W.', 'year': '1967'} / Poly‐α‐Amino Acids by Englander S. W. (1967)

Dates

Type	When
Created	20 years, 8 months ago (Dec. 29, 2004, 7:29 p.m.)
Deposited	1 year, 10 months ago (Oct. 19, 2023, 8:29 a.m.)
Indexed	4 months, 2 weeks ago (April 17, 2025, 7:04 p.m.)
Issued	56 years, 6 months ago (March 1, 1969)
Published	56 years, 6 months ago (March 1, 1969)
Published Online	21 years, 7 months ago (Feb. 1, 2004)
Published Print	56 years, 6 months ago (March 1, 1969)

Funders 0

None

BibTeX

@article{Englander_1969, title={Hydrogen‐tritium exchange of the random chain polypeptide}, volume={7}, ISSN={1097-0282}, url={http://dx.doi.org/10.1002/bip.1969.360070309}, DOI={10.1002/bip.1969.360070309}, number={3}, journal={Biopolymers}, publisher={Wiley}, author={Englander, S. W. and Poulsen, A.}, year={1969}, month=mar, pages={379–393} }

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