DOI: 10.1002/bip.1967.360051009. Thermal transition of DNA measured by NMR spin‐echo technique

Thermal transition of DNA measured by NMR spin‐echo technique

10.1002/bip.1967.360051009

Crossref journal-article

Wiley

Biopolymers (311)

Abstract

AbstractThe thermal helix–coil transition of DNA can be studied by means of the spin‐echo technique. The longitudinal spin–lattice relaxation time T1 and the transvense spin–spin relaxation time T2 of the DNA sample show a similar phase transition as observed spec‐trophotometrically with increasing and decreasing temperatures. Four slopes on the T1 and T2 temperature relationship curves were found and interpreted as functions of nonrelational hydration of the DNA molecule. The T1 and T2 values differ depending on the native or denatured state of the DNA molecule. The importance of the dynamic equilibrium between water molecules in the hydration lattice and steps in the denaturation of the DNA molecule are discussed. This phenomenon may be directly related to the nonrotational hydration.

Bibliography

Lubas, B., Wilczok, T., & K.âDaszkiewicz, O. (1967). Thermal transition of DNA measured by NMR spinâecho technique. Biopolymers, 5(10), 967â974. Portico.

Authors 3

Barbara Lubas (first)
Tadeusz Wilczok (additional)
Olgierd K.‐Daszkiewicz (additional)

References 25 Referenced 11

10.1016/0926-6585(66)90309-8
10.1016/S0022-2836(62)80066-7
10.1073/pnas.44.5.432
{'key': 'e_1_2_1_5_2', 'first-page': '1', 'volume': '12', 'author': 'Doty P.', 'year': '1959', 'journal-title': 'Brookhaven Symp. Biol.'} / Brookhaven Symp. Biol. by Doty P. (1959)
O. K.‐Daszkiewicz Thesis Institute of Nuclear Physics Cracow 1965.
10.1103/PhysRev.94.630
10.1063/1.1716296
A.Abragam The Principles of Nuclear Magnetism Oxford 1961. (10.1063/1.3057238)
{'key': 'e_1_2_1_10_2', 'volume-title': 'Kerninduction', 'author': 'Lösche A.', 'year': '1957'} / Kerninduction by Lösche A. (1957)
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10.1126/science.144.3623.1234
10.1002/bip.1964.360020208
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10.1039/df9582500138
10.1016/0003-9861(62)90110-8
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{'key': 'e_1_2_1_21_2', 'first-page': '234', 'volume': '112', 'author': 'Lewin S.', 'year': '1965', 'journal-title': 'Arch. Biochem. Phys.'} / Arch. Biochem. Phys. by Lewin S. (1965)
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{'key': 'e_1_2_1_23_2', 'first-page': '824', 'volume': '13', 'author': 'Lewin S.', 'year': '1964', 'journal-title': 'Lab. Practice'} / Lab. Practice by Lewin S. (1964)
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10.1016/S0003-9861(66)81038-X

Dates

Type	When
Created	20 years, 7 months ago (Dec. 29, 2004, 5:02 a.m.)
Deposited	1 year, 9 months ago (Nov. 12, 2023, 5:21 a.m.)
Indexed	1 year, 9 months ago (Nov. 12, 2023, 9:52 a.m.)
Issued	57 years, 10 months ago (Oct. 1, 1967)
Published	57 years, 10 months ago (Oct. 1, 1967)
Published Online	21 years, 6 months ago (Feb. 1, 2004)
Published Print	57 years, 10 months ago (Oct. 1, 1967)

Funders 0

None

BibTeX

@article{Lubas_1967, title={Thermal transition of DNA measured by NMR spin‐echo technique}, volume={5}, ISSN={1097-0282}, url={http://dx.doi.org/10.1002/bip.1967.360051009}, DOI={10.1002/bip.1967.360051009}, number={10}, journal={Biopolymers}, publisher={Wiley}, author={Lubas, Barbara and Wilczok, Tadeusz and K.‐Daszkiewicz, Olgierd}, year={1967}, month=oct, pages={967–974} }

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