Infrared absorption spectrum of keratin. I. Spectra of α‐, β‐, and supercontracted keratin
10.1002/bip.1966.360040506
Crossref journal-article
Wiley
Biopolymers (311)
Abstract

AbstractA number of basic features of the infrared spectrum of keratin have been confirmed and some new features have been found. In the 3‐μ region, the amide A frequency of helical material in α‐keratin at 3286 cm.−1 is close to the expected value, but that of the crystalline phase in α‐keratin, near 3270 cm.−1, is lower than had previously been reported. The noncrystalline phase absorbs in the vicinity of 3300 cm.−1 or above, and this causes the low‐intensity component of the amide A band in both α‐ and β‐keratin to occur at higher frequencies than those of the high‐intensity component. In the 6‐μ region, the amide II frequency of noncrystalline material is below 1525 cm.−1. Keratin denatured in lithium bromide, after washing out the reagent, appears to have a considerable helix content, possibly as much as that of the original protein. Hydration causes significant spectral changes. In the 6‐mu; region, the frequency of the amide I band of crystalline material is lowered, while that of the amide II band is increased, both by a few wavenumbers; the amide II frequency of noncrystaline material is also increased by a few wavenumbers. In the 3‐μ region, no significant change is observed in the amide A frequency of crystalline material, while the frequency of the noncrystaline material is reduced. These spectral changes are interpreted in terms of a weak association of water with main‐chain carbonyl groups in the crystalline phase, while in the noncrystaline phase it is thought likely that water molecules form hydrogen‐bond bridges between polypetide chains. The absorption coefficient of the amide A band and the integrated absorption intensities of the amide A, I, and II bands do not vary appreciably in the three forms of keratin investigated.

Bibliography

Bendit, E. G. (1966). Infrared absorption spectrum of keratin. I. Spectra of α‐, β‐, and supercontracted keratin. Biopolymers, 4(5), 539–559. Portico.

Authors 1
  1. E. G. Bendit (first)
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Dates
Type When
Created 20 years, 7 months ago (Dec. 29, 2004, 4:56 a.m.)
Deposited 1 year, 9 months ago (Nov. 12, 2023, 8:57 a.m.)
Indexed 2 weeks, 2 days ago (Aug. 7, 2025, 4:30 p.m.)
Issued 59 years, 3 months ago (May 1, 1966)
Published 59 years, 3 months ago (May 1, 1966)
Published Online 21 years, 6 months ago (Feb. 1, 2004)
Published Print 59 years, 3 months ago (May 1, 1966)
Funders 0

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@article{Bendit_1966, title={Infrared absorption spectrum of keratin. I. Spectra of α‐, β‐, and supercontracted keratin}, volume={4}, ISSN={1097-0282}, url={http://dx.doi.org/10.1002/bip.1966.360040506}, DOI={10.1002/bip.1966.360040506}, number={5}, journal={Biopolymers}, publisher={Wiley}, author={Bendit, E. G.}, year={1966}, month=may, pages={539–559} }