DOI: 10.1002/bip.1966.360040204. Kinetics of renaturation of denatured DNA. I. Spectrophotometric results

Kinetics of renaturation of denatured DNA. I. Spectrophotometric results

10.1002/bip.1966.360040204

Crossref journal-article

Wiley

Biopolymers (311)

Abstract

AbstractThe kinetics of renaturation of heat‐ or formamide‐denatured DNA have been studied by following the change of optical density at a constant temperature. Solvents of different ionic strength and various DNA samples have been used. At the lower ionic strengths studied, the reaction follows second‐order kinetics, substantiating the hypothesis that strands of native DNA separate upon denaturation and recombine during renaturation. As the ionic strength is increased at a constant temperature, the reaction deviates from simple second‐order behavior. This appears to be the result of the inhibition to rewinding caused by short helical segments in the denatured DNA which are more stable at the higher ionic strenth.

Bibliography

Subirana, J. A., & Doty, P. (1966). Kinetics of renaturation of denatured DNA. I. Spectrophotometric results. Biopolymers, 4(2), 171â187. Portico.

Authors 2

Juan A. Subirana (first)
Paul Doty (additional)

References 30 Referenced 51

10.1073/pnas.46.4.453
10.1073/pnas.46.4.461
10.1016/S0022-2836(61)80023-5
10.1002/bip.1966.360040205
10.1016/S0022-2836(61)80047-8
10.1016/0003-2697(60)90020-8
10.1021/bi00906a005
Wierzchowski K. L. unpublished results.
10.1016/0006-3002(61)91013-7
Hearst J. Thesis California Institute of Technology 1961.
Studier F. W. Thesis California Institute of Technology 1963.
10.1016/0005-2787(65)90538-1
10.1016/S0022-2836(64)80142-X
10.1016/S0022-2836(61)80070-3
{'key': 'e_1_2_1_15_3', 'volume-title': 'Informational Macromolecules', 'author': 'Thomas C. A.', 'year': '1963'} / Informational Macromolecules by Thomas C. A. (1963)
10.1016/S0022-2836(65)80007-9
Kohn K. private communication.
10.1073/pnas.43.7.581
10.1021/j100825a011
Schildkraut C. unpublished results.
10.1021/ja01475a039
10.1073/pnas.46.10.1360
10.1016/S0006-3495(62)86859-3
{'key': 'e_1_2_1_24_2', 'first-page': '44', 'volume-title': 'The Molecular Basis of Neoplasia', 'author': 'Cavalieri L. F.', 'year': '1962'} / The Molecular Basis of Neoplasia by Cavalieri L. F. (1962)
Rownd R. Thesis Harvard University 1963.
Lanyi J. Thesis Harvard University 1963.
10.1002/pol.1961.1204915106
10.1016/0006-291X(60)90248-5
10.1016/0006-291X(63)90336-X
10.1016/0006-3002(61)91028-9

Dates

Type	When
Created	20 years, 8 months ago (Dec. 29, 2004, 4:55 a.m.)
Deposited	1 year, 9 months ago (Nov. 12, 2023, 8:51 a.m.)
Indexed	1 year, 7 months ago (Jan. 11, 2024, 11:57 a.m.)
Issued	59 years, 6 months ago (Feb. 1, 1966)
Published	59 years, 6 months ago (Feb. 1, 1966)
Published Online	21 years, 6 months ago (Feb. 1, 2004)
Published Print	59 years, 6 months ago (Feb. 1, 1966)

Funders 0

None

BibTeX

@article{Subirana_1966, title={Kinetics of renaturation of denatured DNA. I. Spectrophotometric results}, volume={4}, ISSN={1097-0282}, url={http://dx.doi.org/10.1002/bip.1966.360040204}, DOI={10.1002/bip.1966.360040204}, number={2}, journal={Biopolymers}, publisher={Wiley}, author={Subirana, Juan A. and Doty, Paul}, year={1966}, month=feb, pages={171–187} }

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