DOI: 10.1002/bies.950150107. Interaction of rhodopsin with the G‐protein, transducin

Interaction of rhodopsin with the G‐protein, transducin

10.1002/bies.950150107

Crossref journal-article

Wiley

BioEssays (311)

Abstract

AbstractRhodopsin, upon activation by light, transduces the photon signal by activation of the G‐protein, transducin. The well‐studied rhodopsin/transducin system serves as a model for the understanding of signal transduction by the large class of G‐protein‐coupled receptors. The interactive form of rhodopsin, R*, is conformationally similar or identical to rhodopsin's photolysis intermediate Metarhodopsin II (MII). Formation of MII requires deprotonation of rhodopsin's protonated Schiff base which appears to facilitate some opening of the rhodopsin structure. This allows a change in conformation at rhodopsin's cytoplasmic surface that provides binding sites for transducin. Rhodopsin's 2nd, 3rd and putative 4th cytoplasmic loops bind transducin at sites including transducin's 5 kDa carboxyl‐terminal region. Site‐specific mutagenesis of rhodopsin is being used to distinguish sites on rhodopsin's surface that are important in binding transducin from those that function in activating transducin. These observations are consistent with and extend studies on the action of other G‐protein‐coupled receptors and their interactions with their respective G proteins.

Bibliography

Hargrave, P. A., Hamm, H. E., & Hofmann, K. P. (1993). Interaction of rhodopsin with the Gâprotein, transducin. BioEssays, 15(1), 43â50. Portico.

Authors 3

Paul A. Hargrave (first)
Heidi E. Hamm (additional)
K. P. Hofmann (additional)

References 66 Referenced 107

10.1016/0042-6989(86)90115-X
10.1096/fasebj.6.6.1544542
10.1096/fasebj.4.14.2172060 / FASEB J. / Transduction of receptor signal into modulation of effector activity by G proteins: the first 20 years or so by Birnbaumer L. (1990)
10.1021/bi00430a052
10.1016/0014-5793(82)80266-4
10.1073/pnas.83.12.4209
10.1016/S0006-3495(91)82279-2
10.1021/bi00241a019
10.1021/bi00440a036
10.1126/science.1990431
{'key': 'e_1_2_1_12_2', 'article-title': 'Constitutive activation of rhodopsin by mutation of lys‐296', 'volume': '6', 'author': 'Robinson P. R.', 'year': '1992', 'journal-title': 'FASEB J.'} / FASEB J. / Constitutive activation of rhodopsin by mutation of lys‐296 by Robinson P. R. (1992)
10.1126/science.2573154
10.1073/pnas.86.21.8309
10.1021/bi00456a013
10.1016/S0021-9258(19)49592-6 / J. Biol. Chem. / The role of arrestin and retinoids in the regeneration pathway of rhodopsin by Hofmann K. P. (1992)
10.1111/j.1432-1033.1989.tb15068.x
10.1016/S0021-9258(17)30605-1 / J. Biol. Chem. / Displacement of rhodopsin by GDP from three loop interaction with transducin depends critically on the diphosphate beta;‐position by Kahlert M. (1990)
10.1038/348125a0
10.1038/349117a0
10.1126/science.3898365
10.1126/science.2406906
10.1002/j.1460-2075.1990.tb07409.x / EMBO J. / Refined crystal structure of the triphosphate conformation of H‐ras p21 at 1.35 A resolution: implications for the mechanism of GTP hydrolysis by Pai E. F. (1990)
10.1093/protein/1.1.47
10.1016/S0021-9258(18)61040-3 / J. Biol. Chem. / Topographic analysis of antigenic determinants recognized by monoclonal antibodies to the photoreceptor guanyl nucleotide‐binding protein, transducin by Deretic D. (1987)
10.1016/0014-5793(87)80867-0
10.1073/pnas.86.6.1751
10.1038/357563a0
10.1073/pnas.86.19.7407
10.1126/science.1902986
{'key': 'e_1_2_1_31_2', 'first-page': '1004', 'article-title': 'Retinal rods and cones have distinct G‐protein γ subunits', 'volume': '33', 'author': 'Yau K.‐W.', 'year': '1992', 'journal-title': 'Invest. Ophthalmol. & Vis. Sci.'} / Invest. Ophthalmol. & Vis. Sci. / Retinal rods and cones have distinct G‐protein γ subunits by Yau K.‐W. (1992)
10.1016/S0021-9258(18)55479-X / J. Biol. Chem. / Amino acid and cDNA sequence of bovine phosducin, a soluble phosphoprotein from photoreceptor cells by Lee R. H. (1990)
{'key': 'e_1_2_1_33_2', 'first-page': '1005', 'article-title': 'Cross‐linking studies of complex formation between phosphorylated Components I and II and G protein in frog rod outer segments', 'volume': '32', 'author': 'Suh K.‐H.', 'year': '1991', 'journal-title': 'Invest. Ophthalmol. & Vis. Sci.'} / Invest. Ophthalmol. & Vis. Sci. / Cross‐linking studies of complex formation between phosphorylated Components I and II and G protein in frog rod outer segments by Suh K.‐H. (1991)
10.1016/S0021-9258(18)45865-6 / J. Biol. Chem. / Activation by G‐protein beta;γ‐subunits of agonist‐dependent or light‐dependent phosphorylation of muscarinic acetylcholine receptors and rhodopsin by Haga K. (1992)
10.1126/science.1962211
10.1038/356159a0
10.1126/science.1317058
10.1042/bj2830273
10.1073/pnas.87.2.728
10.1073/pnas.87.2.568
10.1016/S0021-9258(19)36955-8 / J. Biol. Chem. / The rod transducin alpha subunit amino terminus is heterogeneously fatty acylated by Neubert T. A. (1992)
10.1016/S0021-9258(18)47791-5 / J. Biol. Chem. / Characterization of transducin from bovine retinal rod outer segments by Navon S. E. (1987)
10.1016/S0021-9258(18)31544-8 / J. Biol. Chem. / Deletion within the amino‐terminal region of Gsα impairs its ability to interact with beta;γ subunits and to activate adenylate cyclase by Journot L. (1991)
10.1016/S0021-9258(18)42691-9 / J. Biol. Chem. / Mutagenesis of the amino terminus of the α‐subunit of the G‐protein Go ‐ invitro characterization of αoβγ‐interactions by Denker B. M. (1992)
10.1016/0092-8674(90)90136-3
10.1038/346658a0
10.1016/S0021-9258(18)98880-0 / J. Biol. Chem. / Rhodopsin and the retinal G‐protein distinguish among G‐protein beta;γ subunit forms by Fawzi A. B. (1991)
10.1038/330758a0
10.1016/S0021-9258(18)92811-5 / J. Biol. Chem. / Structural analysis of rod GTP‐binding protein, Gt. Limited proteolytic digestion pattern of Gt with four proteases defines monoclonal antibody epitope by Mazzoni M. R. (1991)
10.1126/science.3136547
10.1021/bi00114a005
{'key': 'e_1_2_1_52_2', 'first-page': '76', 'volume-title': 'The Biology and Medicine of Signal Transduction', 'author': 'Hamm H. E.', 'year': '1990'} / The Biology and Medicine of Signal Transduction by Hamm H. E. (1990)
10.1016/0014-5793(91)81300-W
10.1016/S0021-9258(17)30604-X / J. Biol. Chem. / The effect of GDP on rod outer segment G‐protein interactions by Panico J. (1990)
{'key': 'e_1_2_1_55_2', 'first-page': '872', 'article-title': '2D‐NMR studies of the three‐dimensional structure of Gαt carboxyl terminal peptides in the free and rhodopsin‐bound conformations', 'volume': '33', 'author': 'Dratz E.', 'year': '1992', 'journal-title': 'Invest. Ophthalmol. & Vis. Sci.'} / Invest. Ophthalmol. & Vis. Sci. / 2D‐NMR studies of the three‐dimensional structure of Gαt carboxyl terminal peptides in the free and rhodopsin‐bound conformations by Dratz E. (1992)
{'key': 'e_1_2_1_56_2', 'first-page': '356', 'article-title': 'Effects of site‐specific peptides on blocking receptor‐G protein interaction in permeable C6 cells', 'volume': '17', 'author': 'Lazarevic M. B.', 'year': '1991', 'journal-title': 'Soc. Neurosci. Abstr.'} / Soc. Neurosci. Abstr. / Effects of site‐specific peptides on blocking receptor‐G protein interaction in permeable C6 cells by Lazarevic M. B. (1991)
{'key': 'e_1_2_1_57_2', 'first-page': '356', 'article-title': 'Site‐specific Gs peptides evoke high affinity ligand binding to beta;‐adrenergic receptors in permeable cells', 'volume': '17', 'author': 'Watanabe M.', 'year': '1991', 'journal-title': 'Soc. Neurosci. Abstr.'} / Soc. Neurosci. Abstr. / Site‐specific Gs peptides evoke high affinity ligand binding to beta;‐adrenergic receptors in permeable cells by Watanabe M. (1991)
10.1021/bi00512a007
{'key': 'e_1_2_1_59_2', 'first-page': '345', 'article-title': 'The cyclic GMP enzyme cascade of vision: site of light activation localized by enzymatic modifications of rhodopsin', 'volume': '62', 'author': 'Wehner M.', 'year': '1987', 'journal-title': 'Advances in the Biosciences'} / Advances in the Biosciences / The cyclic GMP enzyme cascade of vision: site of light activation localized by enzymatic modifications of rhodopsin by Wehner M. (1987)
10.1073/pnas.86.18.6878
10.1016/S0021-9258(18)69178-1 / J. Biol. Chem. / A single amino acid substitution in rhodopsin (Lysine 248 ‐ Leucine) prevents activation of transducin by Franke R. H. (1988)
10.1126/science.2218504
10.1016/S0959-440X(05)80080-6
10.1016/S0021-9258(18)37546-X / J. Biol. Chem / Site‐directed mutagenesis of the cytoplasmic domains of the human beta;2‐adrenergic receptor. Localization of regions involved in G protein‐receptor coupling by O'Dowd D. F. (1988)
{'key': 'e_1_2_1_65_2', 'first-page': '517', 'article-title': 'Delineation of muscarinic receptor domains conferring selectivity of coupling to guanine nucleotide‐binding proteins and second messengers', 'volume': '38', 'author': 'Wess J.', 'year': '1990', 'journal-title': 'Molec. Pharmacol.'} / Molec. Pharmacol. / Delineation of muscarinic receptor domains conferring selectivity of coupling to guanine nucleotide‐binding proteins and second messengers by Wess J. (1990)
10.1002/j.1460-2075.1990.tb07888.x
10.1016/S0021-9258(18)45962-5 / J. Biol. Chem. / Constitutive activation of the alpha‐1B‐adrenergic receptor by all amino acid substitutions at a single site ‐ evidence for a region which constrains receptor activation by Kjelsberg M. A. (1992)

Dates

Type	When
Created	20 years, 6 months ago (Feb. 25, 2005, 5:40 a.m.)
Deposited	1 year, 10 months ago (Oct. 24, 2023, 5:17 p.m.)
Indexed	19 minutes ago (Sept. 2, 2025, 8:29 p.m.)
Issued	32 years, 8 months ago (Jan. 1, 1993)
Published	32 years, 8 months ago (Jan. 1, 1993)
Published Online	20 years, 6 months ago (Feb. 5, 2005)
Published Print	32 years, 8 months ago (Jan. 1, 1993)

Funders 0

None

BibTeX

@article{Hargrave_1993, title={Interaction of rhodopsin with the G‐protein, transducin}, volume={15}, ISSN={1521-1878}, url={http://dx.doi.org/10.1002/bies.950150107}, DOI={10.1002/bies.950150107}, number={1}, journal={BioEssays}, publisher={Wiley}, author={Hargrave, Paul A. and Hamm, Heidi E. and Hofmann, K. P.}, year={1993}, month=jan, pages={43–50} }

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