DOI: 10.1002/bies.950131009. Transfer of yeast artificial chromosomes from yeast to mammalian cells

Transfer of yeast artificial chromosomes from yeast to mammalian cells

10.1002/bies.950131009

Crossref journal-article

Wiley

BioEssays (311)

Abstract

AbstractHuman DNA can be cloned as yeast artificial chromosomes (YACs), each of which contains several hundred kilobases of human DNA. This DNA can be manipulated in the yeast host using homologous recombination and yeast selectable markers. In relatively few steps it is possible to make virtually any change in the cloned human DNA from single base pair changes to deletions and insertions. In order to study the function of the cloned DNA and the effects of the changes made in the yeast, the human DNA must be transferred back into mammalian cells. Recent experiments indicate that large genes can be transferred from the yeast host to mammalian cells in tissue culture and that the genes are transferred intact and are expressed. Using the same methods it may soon be possible to transfer YAC DNA into the mouse germ line so that the expression and function of genes cloned in YACs can be studied in developing and adult mammalian animals.

Bibliography

Huxley, C., & Gnirke, A. (1991). Transfer of yeast artificial chromosomes from yeast to mammalian cells. BioEssays, 13(10), 545â550. Portico.

Authors 2

Clare Huxley (first)
Andreas Gnirke (additional)

References 40 Referenced 16

10.1126/science.3033825
10.1073/pnas.87.11.4256
10.1093/nar/18.8.1951
10.1016/0888-7543(90)90285-3
{'key': 'e_1_2_1_6_2', 'first-page': '95', 'article-title': 'Human Xq24‐Xq28: approaches to mapping with yeast artificial chromosomes', 'volume': '46', 'author': 'Wada M.', 'year': '1990', 'journal-title': 'Am. J. Hum. Genet.'} / Am. J. Hum. Genet. / Human Xq24‐Xq28: approaches to mapping with yeast artificial chromosomes by Wada M. (1990)
10.1126/science.2218515
10.1016/0888-7543(91)90229-8
10.1016/0888-7543(91)90245-A
{'key': 'e_1_2_1_10_2', 'first-page': '281', 'volume-title': 'Methods in Enzymology', 'author': 'Rothstein R.', 'year': '1991'} / Methods in Enzymology by Rothstein R. (1991)
10.1016/S0092-8674(85)80117-3
{'key': 'e_1_2_1_12_2', 'first-page': '202', 'volume-title': 'Methods in Enzymology', 'author': 'Rothstein R. J.', 'year': '1983'} / Methods in Enzymology by Rothstein R. J. (1983)
10.1002/yea.1478
10.1073/pnas.87.4.1300
10.1073/pnas.87.24.9913
10.1016/0888-7543(91)90369-P
10.1002/j.1460-2075.1991.tb07685.x
10.1073/pnas.88.6.2179
10.1073/pnas.87.13.5109
10.1128/MCB.10.8.4163
10.1007/BF01560657
10.1016/0092-8674(87)90493-4
10.1073/pnas.86.15.5898
10.1016/0022-2836(79)90267-5
10.3109/10409238409108719
{'key': 'e_1_2_1_26_2', 'first-page': '988', 'article-title': 'Efficient transformation and frequent single‐site, single‐copy insertion of DNA can be obtained in mouse erythroleukemia cells transformed by electroporation', 'volume': '14', 'author': 'Boggs S. S.', 'year': '1986', 'journal-title': 'Exp. Hematol'} / Exp. Hematol / Efficient transformation and frequent single‐site, single‐copy insertion of DNA can be obtained in mouse erythroleukemia cells transformed by electroporation by Boggs S. S. (1986)
10.1093/nar/17.9.3425
10.1093/nar/17.19.8010
10.1073/pnas.87.21.8242
10.1073/pnas.77.12.7380
10.1038/323445a0
10.1038/338150a0
10.1016/0166-6851(91)90006-R
10.1073/pnas.87.23.9358
10.1073/pnas.87.1.103
10.1146/annurev.ge.20.120186.002341
10.1016/0092-8674(87)90584-8
10.1016/0092-8674(91)90318-S
10.1038/341343a0
10.1038/350243a0
10.1093/nar/18.24.7349

Dates

Type	When
Created	20 years, 6 months ago (Feb. 25, 2005, 5:30 a.m.)
Deposited	1 year, 10 months ago (Oct. 23, 2023, 11:52 a.m.)
Indexed	1 year, 10 months ago (Oct. 24, 2023, 7:41 a.m.)
Issued	33 years, 11 months ago (Oct. 1, 1991)
Published	33 years, 11 months ago (Oct. 1, 1991)
Published Online	20 years, 6 months ago (Feb. 5, 2005)
Published Print	33 years, 11 months ago (Oct. 1, 1991)

Funders 0

None

BibTeX

@article{Huxley_1991, title={Transfer of yeast artificial chromosomes from yeast to mammalian cells}, volume={13}, ISSN={1521-1878}, url={http://dx.doi.org/10.1002/bies.950131009}, DOI={10.1002/bies.950131009}, number={10}, journal={BioEssays}, publisher={Wiley}, author={Huxley, Clare and Gnirke, Andreas}, year={1991}, month=oct, pages={545–550} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2023,
        10,
        24
      ]
    ],
    "date-time": "2023-10-24T11:41:03Z",
    "timestamp": 1698147663287
  },
  "reference-count": 40,
  "publisher": "Wiley",
  "issue": "10",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2005,
            2,
            5
          ]
        ],
        "date-time": "2005-02-05T00:00:00Z",
        "timestamp": 1107561600000
      },
      "content-version": "vor",
      "delay-in-days": 4876,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [
      "onlinelibrary.wiley.com"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1991,
        10
      ]
    ]
  },
  "abstract": "<jats:title>Abstract</jats:title><jats:p>Human DNA can be cloned as yeast artificial chromosomes (YACs), each of which contains several hundred kilobases of human DNA. This DNA can be manipulated in the yeast host using homologous recombination and yeast selectable markers. In relatively few steps it is possible to make virtually any change in the cloned human DNA from single base pair changes to deletions and insertions. In order to study the function of the cloned DNA and the effects of the changes made in the yeast, the human DNA must be transferred back into mammalian cells. Recent experiments indicate that large genes can be transferred from the yeast host to mammalian cells in tissue culture and that the genes are transferred intact and are expressed. Using the same methods it may soon be possible to transfer YAC DNA into the mouse germ line so that the expression and function of genes cloned in YACs can be studied in developing and adult mammalian animals.</jats:p>",
  "DOI": "10.1002/bies.950131009",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2005,
        2,
        25
      ]
    ],
    "date-time": "2005-02-25T10:30:37Z",
    "timestamp": 1109327437000
  },
  "page": "545-550",
  "update-policy": "http://dx.doi.org/10.1002/crossmark_policy",
  "source": "Crossref",
  "is-referenced-by-count": 16,
  "title": "Transfer of yeast artificial chromosomes from yeast to mammalian cells",
  "prefix": "10.1002",
  "volume": "13",
  "author": [
    {
      "given": "Clare",
      "family": "Huxley",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Andreas",
      "family": "Gnirke",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        2005,
        2,
        5
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_1_2_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.3033825"
    },
    {
      "key": "e_1_2_1_3_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.87.11.4256"
    },
    {
      "key": "e_1_2_1_4_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/nar/18.8.1951"
    },
    {
      "key": "e_1_2_1_5_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0888-7543(90)90285-3"
    },
    {
      "key": "e_1_2_1_6_2",
      "first-page": "95",
      "article-title": "Human Xq24\u2010Xq28: approaches to mapping with yeast artificial chromosomes",
      "volume": "46",
      "author": "Wada M.",
      "year": "1990",
      "journal-title": "Am. J. Hum. Genet."
    },
    {
      "key": "e_1_2_1_7_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.2218515"
    },
    {
      "key": "e_1_2_1_8_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0888-7543(91)90229-8"
    },
    {
      "key": "e_1_2_1_9_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0888-7543(91)90245-A"
    },
    {
      "key": "e_1_2_1_10_2",
      "first-page": "281",
      "volume-title": "Methods in Enzymology",
      "author": "Rothstein R.",
      "year": "1991"
    },
    {
      "key": "e_1_2_1_11_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(85)80117-3"
    },
    {
      "key": "e_1_2_1_12_2",
      "first-page": "202",
      "volume-title": "Methods in Enzymology",
      "author": "Rothstein R. J.",
      "year": "1983"
    },
    {
      "key": "e_1_2_1_13_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/yea.1478"
    },
    {
      "key": "e_1_2_1_14_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.87.4.1300"
    },
    {
      "key": "e_1_2_1_15_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.87.24.9913"
    },
    {
      "key": "e_1_2_1_16_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0888-7543(91)90369-P"
    },
    {
      "key": "e_1_2_1_17_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/j.1460-2075.1991.tb07685.x"
    },
    {
      "key": "e_1_2_1_18_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.88.6.2179"
    },
    {
      "key": "e_1_2_1_19_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.87.13.5109"
    },
    {
      "key": "e_1_2_1_20_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/MCB.10.8.4163"
    },
    {
      "key": "e_1_2_1_21_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF01560657"
    },
    {
      "key": "e_1_2_1_22_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(87)90493-4"
    },
    {
      "key": "e_1_2_1_23_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.86.15.5898"
    },
    {
      "key": "e_1_2_1_24_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0022-2836(79)90267-5"
    },
    {
      "key": "e_1_2_1_25_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.3109/10409238409108719"
    },
    {
      "key": "e_1_2_1_26_2",
      "first-page": "988",
      "article-title": "Efficient transformation and frequent single\u2010site, single\u2010copy insertion of DNA can be obtained in mouse erythroleukemia cells transformed by electroporation",
      "volume": "14",
      "author": "Boggs S. S.",
      "year": "1986",
      "journal-title": "Exp. Hematol"
    },
    {
      "key": "e_1_2_1_27_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/nar/17.9.3425"
    },
    {
      "key": "e_1_2_1_28_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/nar/17.19.8010"
    },
    {
      "key": "e_1_2_1_29_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.87.21.8242"
    },
    {
      "key": "e_1_2_1_30_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.77.12.7380"
    },
    {
      "key": "e_1_2_1_31_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/323445a0"
    },
    {
      "key": "e_1_2_1_32_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/338150a0"
    },
    {
      "key": "e_1_2_1_33_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0166-6851(91)90006-R"
    },
    {
      "key": "e_1_2_1_34_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.87.23.9358"
    },
    {
      "key": "e_1_2_1_35_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.87.1.103"
    },
    {
      "key": "e_1_2_1_36_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1146/annurev.ge.20.120186.002341"
    },
    {
      "key": "e_1_2_1_37_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(87)90584-8"
    },
    {
      "key": "e_1_2_1_38_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(91)90318-S"
    },
    {
      "key": "e_1_2_1_39_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/341343a0"
    },
    {
      "key": "e_1_2_1_40_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/350243a0"
    },
    {
      "key": "e_1_2_1_41_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/nar/18.24.7349"
    }
  ],
  "container-title": "BioEssays",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fbies.950131009",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://onlinelibrary.wiley.com/doi/pdf/10.1002/bies.950131009",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        10,
        23
      ]
    ],
    "date-time": "2023-10-23T15:52:53Z",
    "timestamp": 1698076373000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://onlinelibrary.wiley.com/doi/10.1002/bies.950131009"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1991,
        10
      ]
    ]
  },
  "references-count": 40,
  "journal-issue": {
    "issue": "10",
    "published-print": {
      "date-parts": [
        [
          1991,
          10
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1002/bies.950131009"
  ],
  "URL": "http://dx.doi.org/10.1002/bies.950131009",
  "relation": {},
  "ISSN": [
    "0265-9247",
    "1521-1878"
  ],
  "subject": [],
  "container-title-short": "BioEssays",
  "published": {
    "date-parts": [
      [
        1991,
        10
      ]
    ]
  },
  "assertion": [
    {
      "value": "2005-02-05",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}