DOI: 10.1002/bies.10233. Nonculturable bacteria: programmed survival forms or cells at death's door?

Nonculturable bacteria: programmed survival forms or cells at death's door?

10.1002/bies.10233

Crossref journal-article

Wiley

BioEssays (311)

Abstract

AbstractUpon starvation and growth arrest, Escherichia coli cells gradually lose their ability to reproduce. These apparently sterile/nonculturable cells initially remain intact and metabolically active and the underlying molecular mechanism behind this sterility is something of an enigma in bacteriology. Three different models have been proposed to explain this phenomenon. The first theory suggests that starving cells become nonculturable due to cellular deterioration, are moribund, and show some of the same signs of senescence as aging organisms. The two other theories suggest that genetically programmed pathways, rather than stochastic deterioration, trigger nonculturability. One “program” theory suggests that nonculturability is the culmination of an adaptive pathway generating dormant survival forms, similar to spore formation in differentiating bacteria. The other “program” theory states that starved cells lose viability due to activation of genetic modules mediating programmed cell death. The different models will be reviewed and evaluated in light of recent data on the physiology and molecular biology of growth‐arrested E. coli cells. BioEssays 25:204–211, 2003. © 2003 Wiley Periodicals, Inc.

Bibliography

NystrÃ¶m, T. (2003). Nonculturable bacteria: programmed survival forms or cells at deathâs door? BioEssays, 25(3), 204â211. Portico.

Authors 1

Thomas Nyström (first)

References 57 Referenced 72

{'key': 'e_1_2_1_2_2', 'volume-title': 'General Microbiology', 'author': 'Schlegel H', 'year': '1986'} / General Microbiology by Schlegel H (1986)
10.1042/bj1020236
10.1099/00221287-93-1-1
10.1128/jb.160.3.1041-1046.1984
10.1128/JB.157.3.758-763.1984
10.1111/j.1365-2958.1991.tb01819.x
10.1111/j.1365-2958.1991.tb01825.x
10.1016/0092-8674(93)90655-A
10.1146/annurev.mi.47.100193.004231
10.1002/j.1460-2075.1996.tb00686.x
10.1016/S1369-5274(99)80037-X
10.1016/S1568-1637(02)00028-4
10.1007/BF02010671
10.1139/m79-063
10.1007/978-1-4757-0271-2_1
10.1128/mr.51.3.365-379.1987
10.1007/978-1-4757-0271-2_18
10.1007/978-1-4757-0271-2_17
10.1007/978-1-4757-0271-2_16
10.1016/S0065-2911(08)60166-6
10.1023/A:1000664013047
10.1016/S1369-5274(00)00082-5
10.1128/JB.182.18.5070-5075.2000
10.1093/embo-reports/kve182
10.1111/j.1574-6968.1994.tb07007.x
10.1128/aem.63.3.1002-1005.1997
10.1007/978-1-4615-5925-2_3
10.1016/0022-2836(92)90629-X
10.1016/S0022-2836(05)80138-X
10.1046/j.1365-2958.1998.00993.x
10.1073/pnas.93.12.6059
10.1073/pnas.95.26.15481
10.1128/JB.182.3.561-572.2000
10.1046/j.1365-2958.2002.03027.x / Mol Microbiol / Rapid induction and reversal of a bacteriostatic condition by controlled expression of toxins and antitoxins by Pedersen K (2002)
10.1007/s002030100314
10.1073/pnas.90.19.8905
10.1016/S0531-5565(00)00138-8
10.1093/gerona/54.2.B47
10.1101/gad.12.21.3431
10.1074/jbc.274.37.26027
10.1074/jbc.M111024200
10.1046/j.1365-2958.2002.02787.x
10.1016/S0021-9258(19)61523-1
10.1073/pnas.94.21.11168
10.1074/jbc.273.5.3027
10.1046/j.1365-2249.1999.01009.x
10.1093/emboj/20.18.5280
10.1016/0092-8674(78)90241-6
10.1073/pnas.100422497
10.1016/S0014-5793(97)01570-6
10.1006/jmbi.1996.0565
10.1038/newbio237131a0
10.1128/JB.182.19.5551-5555.2000
10.1128/jb.133.1.75-80.1978 / J Bacteriol / Single‐strand breakage in DNA of Escherichia coli exposed to Cd2+ by Mitra RS (1978)
10.1128/jb.178.3.927-930.1996 / J Bacteriol / Effects of overproducing the universal stress protein, UspA, in Escherichia coli K‐12 by Nyström T (1996)
10.1073/pnas.95.15.8916
{'key': 'e_1_2_1_58_2', 'volume-title': 'The outer reaches of life', 'author': 'Postgate J', 'year': '1994'} / The outer reaches of life by Postgate J (1994)

Dates

Type	When
Created	22 years, 6 months ago (Feb. 21, 2003, 8:59 a.m.)
Deposited	1 year, 10 months ago (Oct. 7, 2023, 11:39 a.m.)
Indexed	1 year, 1 month ago (July 11, 2024, 3:18 a.m.)
Issued	22 years, 6 months ago (Feb. 20, 2003)
Published	22 years, 6 months ago (Feb. 20, 2003)
Published Online	22 years, 6 months ago (Feb. 20, 2003)
Published Print	22 years, 6 months ago (March 1, 2003)

Funders 1

The Swedish Natural Science Council, VR

BibTeX

@article{Nystr_m_2003, title={Nonculturable bacteria: programmed survival forms or cells at death’s door?}, volume={25}, ISSN={1521-1878}, url={http://dx.doi.org/10.1002/bies.10233}, DOI={10.1002/bies.10233}, number={3}, journal={BioEssays}, publisher={Wiley}, author={Nyström, Thomas}, year={2003}, month=feb, pages={204–211} }

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