DOI: 10.1002/ar.1091640403. The paranucleolar structure, accessory body of cajal, sex chromatin, and related structures in nuclei of rat trigeminal neurons: A cytochemical and ultrastructural study

The paranucleolar structure, accessory body of cajal, sex chromatin, and related structures in nuclei of rat trigeminal neurons: A cytochemical and ultrastructural study

10.1002/ar.1091640403

Crossref journal-article

Wiley

The Anatomical Record (311)

Abstract

AbstractComparative light and electron microscopic study of nuclei in rat trigeminal neurons identified two structures which are the ultrastructural equivalent of the paranucloelar structure and the accessory body of Cajal, two nucleoplasmic structures previously demonstrated in other neurons by light microscope silver staining methods. Ultrastructural evidence indicates that the dense component of the nucleolus is converted into the paranucleolar structure, which then detaches from the nucleolar surface to lie free in the nucleoplasm as the accessory body of Cajal. The cytochemistry, ultrastructure, and antimonate reactivity of the paranucleolar structure and accessory body were identical. Both structures lacked cytochemically demonstrable DNA, RNA, or basic protein.The neuronal nuclei also contained Feulgen‐positive sex chromatin bodies that adhered to the nucleolus, the nuclear membrane, or to both of these structures in specimens of female but not male rats. The ultrastructure and antimonate reactivity of these bodies closely resembled that of heterochromatin clumps but differed markedly from that of the paranucleolar structures and accessory bodies.Additional structures characterized ultrastructurally included patches, granular bodies, and flakes. These structures, like the paranucleolar structure and the accessory body of Cajal, are apparently unique to nuclei of neurons. Cytochemical methods showed that the patches contained basic protein but no nucleic acid.

Bibliography

Hardin, J. H., Spicer, S. S., & Greene, W. B. (1969). The paranucleolar structure, accessory body of cajal, sex chromatin, and related structures in nuclei of rat trigeminal neurons: A cytochemical and ultrastructural study. The Anatomical Record, 164(4), 403â431. Portico.

Authors 3

James H. Hardin (first)
Samuel S. Spicer (additional)
William B. Greene (additional)

References 42 Referenced 64

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{'key': 'e_1_2_1_5_1', 'first-page': '541', 'article-title': 'Electron microscopy of the trigeminal ganglion. I. Comparative ultrastructure', 'volume': '79', 'author': 'Beaver D. L.', 'year': '1965', 'journal-title': 'Arch. Pathol.'} / Arch. Pathol. / Electron microscopy of the trigeminal ganglion. I. Comparative ultrastructure by Beaver D. L. (1965)
{'key': 'e_1_2_1_6_1', 'first-page': '557', 'article-title': 'Electron microscopy of the trigeminal ganglion. II. Autopsy study of human ganglia', 'volume': '79', 'author': 'Beaver D. L.', 'year': '1965', 'journal-title': 'Arch. Pathol.'} / Arch. Pathol. / Electron microscopy of the trigeminal ganglion. II. Autopsy study of human ganglia by Beaver D. L. (1965)
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{'key': 'e_1_2_1_18_1', 'first-page': '54A', 'article-title': 'Nucleolar ultrastructure clarified by pyroantimonate‐osmium fixation', 'volume': '35', 'author': 'Hardin J. H.', 'year': '1967', 'journal-title': 'J. Cell. Biol.'} / J. Cell. Biol. / Nucleolar ultrastructure clarified by pyroantimonate‐osmium fixation by Hardin J. H. (1967)
{'key': 'e_1_2_1_19_1', 'first-page': '360', 'article-title': 'Quantitation of nucleolar componenets in rat trigeminal neurons', 'volume': '160', 'author': 'Hardin J. H.', 'year': '1968', 'journal-title': 'Anat. Rec.'} / Anat. Rec. / Quantitation of nucleolar componenets in rat trigeminal neurons by Hardin J. H. (1968)
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Dates

Type	When
Created	20 years, 5 months ago (Feb. 26, 2005, 3:17 a.m.)
Deposited	1 year, 7 months ago (Jan. 23, 2024, 3:50 p.m.)
Indexed	1 month, 2 weeks ago (July 7, 2025, 2:28 a.m.)
Issued	56 years ago (Aug. 1, 1969)
Published	56 years ago (Aug. 1, 1969)
Published Online	20 years, 6 months ago (Feb. 2, 2005)
Published Print	56 years ago (Aug. 1, 1969)

Funders 0

None

BibTeX

@article{Hardin_1969, title={The paranucleolar structure, accessory body of cajal, sex chromatin, and related structures in nuclei of rat trigeminal neurons: A cytochemical and ultrastructural study}, volume={164}, ISSN={1097-0185}, url={http://dx.doi.org/10.1002/ar.1091640403}, DOI={10.1002/ar.1091640403}, number={4}, journal={The Anatomical Record}, publisher={Wiley}, author={Hardin, James H. and Spicer, Samuel S. and Greene, William B.}, year={1969}, month=aug, pages={403–431} }

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