DOI: 10.1002/anie.201609108. Coupling Sensitive Nucleic Acid Amplification with Commercial Pregnancy Test Strips

Coupling Sensitive Nucleic Acid Amplification with Commercial Pregnancy Test Strips

10.1002/anie.201609108

Crossref journal-article

Wiley

Angewandte Chemie International Edition (311)

Abstract

AbstractThe detection of nucleic acid biomarkers for point‐of‐care (POC) diagnostics is currently limited by technical complexity, cost, and time constraints. To overcome these shortcomings, we have combined loop‐mediated isothermal amplification (LAMP), programmable toehold‐mediated strand‐exchange signal transduction, and standard pregnancy test strips. The incorporation of an engineered hCG–SNAP fusion reporter protein (human chorionic gonadotropin‐O6‐alkylguanine‐DNA alkyltransferase) led to LAMP‐to‐hCG signal transduction on low‐cost, commercially available pregnancy test strips. Our assay reliably detected as few as 20 copies of Ebola virus templates in both human serum and saliva and could be adapted to distinguish a common melanoma‐associated SNP allele (BRAF V600E) from the wild‐type sequence. The methods described are completely generalizable to many nucleic acid biomarkers, and could be adapted to provide POC diagnostics for a range of pathogens.

Bibliography

Du, Y., Pothukuchy, A., Gollihar, J. D., Nourani, A., Li, B., & Ellington, A. D. (2016). Coupling Sensitive Nucleic Acid Amplification with Commercial Pregnancy Test Strips. Angewandte Chemie International Edition, 56(4), 992â996. Portico.

Authors 6

Yan Du (first)
Arti Pothukuchy (additional)
Jimmy D. Gollihar (additional)
Armin Nourani (additional)
Bingling Li (additional)
Andrew D. Ellington (additional)

References 24 Referenced 151

10.14219/jada.archive.2006.0180
10.1016/j.cbpa.2006.01.006
10.1007/s002160051549
10.1039/c3cs35528d
10.1038/nature05448
10.1038/ng1002-229
10.1002/anie.201407021
10.1002/ange.201407021
10.1002/anie.201405995
10.1002/ange.201405995
10.1016/j.cca.2009.01.002
10.1038/nchem.1092
10.1021/acs.analchem.5b01085
10.1021/ac4040554
10.1038/srep11039
10.1021/ac504387c
{'key': 'e_1_2_2_15_1', 'volume-title': 'Handbook of Biosensors and Biochips, Part Eight. Biosensor Applications', 'author': 'Davies R. J.', 'year': '2008'} / Handbook of Biosensors and Biochips, Part Eight. Biosensor Applications by Davies R. J. (2008)
10.1016/j.jviromet.2012.11.041
10.1016/j.parint.2014.06.004
10.1006/bbrc.2001.6334
10.1093/nar/28.12.e63
10.1038/ng1054
10.1038/nchem.1713
10.1038/nchem.1246

Dates

Type	When
Created	8 years, 8 months ago (Dec. 19, 2016, 4:53 a.m.)
Deposited	1 year, 11 months ago (Sept. 18, 2023, 4:26 a.m.)
Indexed	4 days, 16 hours ago (Aug. 19, 2025, 6:55 a.m.)
Issued	8 years, 8 months ago (Dec. 19, 2016)
Published	8 years, 8 months ago (Dec. 19, 2016)
Published Online	8 years, 8 months ago (Dec. 19, 2016)
Published Print	8 years, 7 months ago (Jan. 19, 2017)

Funders 0

None

BibTeX

@article{Du_2016, title={Coupling Sensitive Nucleic Acid Amplification with Commercial Pregnancy Test Strips}, volume={56}, ISSN={1521-3773}, url={http://dx.doi.org/10.1002/anie.201609108}, DOI={10.1002/anie.201609108}, number={4}, journal={Angewandte Chemie International Edition}, publisher={Wiley}, author={Du, Yan and Pothukuchy, Arti and Gollihar, Jimmy D. and Nourani, Armin and Li, Bingling and Ellington, Andrew D.}, year={2016}, month=dec, pages={992–996} }

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