Crossref
journal-article
Wiley
Angewandte Chemie International Edition in English (311)
References
29
Referenced
30
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- A sulfide‐containing DNA oligomer of the same sequence as12that incor porates TST dimers10was found to exhibit cooperative binding tobothnatural DNA14and RNA15.Tmvalues of 32 and 30 °C respectively were obtained at 100 mMNaCI.
- S. H.Kawai D.Wang P. A.Giannaris M. J.Damha G.Just Nucl. Acids Res.21 in press.
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/ Oligonucleotide Synthesis: A Practical Approach by Gait M. J. (1984)- SVPDE = snake venom phosphodiesterase CSPDE = calf spleen phosphodiesterase; triol11was incubated with enzyme at 37°C for 36 h after which time the digests were analyzed by HPLC. Under identical conditions TpT was completely degraded to monomers.
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- After cleavage from the support and base‐deprotection the oligomers were detritylated and purified by reverse‐phase chromatography with OPC (Applied Biosystems) cartridges. RNA oligomer15was purified on 16% PAGE and desalted by reverse‐phase chromatography (C18 Sep‐Pack Waters).
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- 2′ 5′‐Linked oligo‐RNA binds RNA selectively over DNA:M. J.Damha P. A.Giannaris unpublished results.
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Dates
Type | When |
---|---|
Created | 21 years, 8 months ago (Dec. 30, 2003, 5:09 p.m.) |
Deposited | 1 year, 10 months ago (Oct. 10, 2023, 11:32 a.m.) |
Indexed | 1 year, 7 months ago (Jan. 18, 2024, 12:01 p.m.) |
Issued | 32 years, 4 months ago (May 1, 1993) |
Published | 32 years, 4 months ago (May 1, 1993) |
Published Online | 21 years, 8 months ago (Dec. 22, 2003) |
Published Print | 32 years, 4 months ago (May 1, 1993) |
@article{Meng_1993, title={A Sulfide‐Linked Oligonucleotide Analogue with Selective Hybridization Properties}, volume={32}, ISSN={0570-0833}, url={http://dx.doi.org/10.1002/anie.199307291}, DOI={10.1002/anie.199307291}, number={5}, journal={Angewandte Chemie International Edition in English}, publisher={Wiley}, author={Meng, Bin and Kawai, Stephen H. and Wang, Daguang and Just, George and Giannaris, Paul A. and Damha, Masad J.}, year={1993}, month=may, pages={729–731} }