DOI: 10.1002/ana.410230405. Tumor necrosis factor mediates myelin and oligodendrocyte damage in vitro

Tumor necrosis factor mediates myelin and oligodendrocyte damage in vitro

10.1002/ana.410230405

Crossref journal-article

Wiley

Annals of Neurology (311)

Abstract

AbstractRecombinant human tumor necrosis factor (rhTNF) has been tested for its effect on myelinated cultures of mouse spinal cord tissue. As controls, recombinant human interferon gamma (rhIFN) and interleukin‐2 (rhIL‐2) were tested, as well as T‐cell supernatants, antigalactocerebroside serum, and normal culture medium. It was found that rhTNF induced delayed‐onset (18–24 hr) oligodendrocyte necrosis and a type of myelin dilatation peculiar to this system. Some nerve fibers progressed to demyelination by 72 hours. The myelin dilatation was not reversible by return to normal feeding solution for 3 days. In contrast, rhIFN, rhIL‐2, T‐cell supernatants, and normal medium had little or no effect on cultures. This mechanism differs from other immune‐mediated mechanisms in that it appears that a physiological (not structural) demyelination occurs initially without overt destruction of the myelin sheath. These observations are relevant to the evolution of the multiple sclerosis plaque: dysfunction of ionic channels might contribute to the eventual demise of oligodendrocytes and axons in the longstanding lesion.

Bibliography

Selmaj, K. W., & Raine, C. S. (1988). Tumor necrosis factor mediates myelin and oligodendrocyte damage in vitro. Annals of Neurology, 23(4), 339â346. Portico.

Authors 2

Krzysztof W. Selmaj (first)
Cedric S. Raine (additional)

References 48 Referenced 1,073

10.4049/jimmunol.136.8.2938 / J Immunol / Tumor necrosis factor induction by Sendai virus by Aderka DJ (1986)
10.1038/318665a0
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10.1126/science.172.3987.995
{'key': 'e_1_2_1_6_2', 'first-page': '248', 'article-title': 'The distribution of interleukin‐2 receptor bearing lymphocytes in multiple sclerosis: evidence for a key role of activated lymphocytes', 'volume': '61', 'author': 'Bellamy AS', 'year': '1985', 'journal-title': 'Clin Exp Immunol'} / Clin Exp Immunol / The distribution of interleukin‐2 receptor bearing lymphocytes in multiple sclerosis: evidence for a key role of activated lymphocytes by Bellamy AS (1985)
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10.1038/318104a0

Dates

Type	When
Created	20 years, 8 months ago (Jan. 1, 2005, 12:57 p.m.)
Deposited	1 year, 10 months ago (Oct. 20, 2023, 10:34 p.m.)
Indexed	9 hours, 46 minutes ago (Sept. 3, 2025, 5:57 a.m.)
Issued	37 years, 5 months ago (April 1, 1988)
Published	37 years, 5 months ago (April 1, 1988)
Published Online	20 years, 10 months ago (Oct. 8, 2004)
Published Print	37 years, 5 months ago (April 1, 1988)

Funders 0

None

BibTeX

@article{Selmaj_1988, title={Tumor necrosis factor mediates myelin and oligodendrocyte damage in vitro}, volume={23}, ISSN={1531-8249}, url={http://dx.doi.org/10.1002/ana.410230405}, DOI={10.1002/ana.410230405}, number={4}, journal={Annals of Neurology}, publisher={Wiley}, author={Selmaj, Krzysztof W. and Raine, Cedric S.}, year={1988}, month=apr, pages={339–346} }

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