DOI: 10.1002/ana.410070211. Immunocytochemical method to identify myelin basic protein in oligodendroglia and myelin sheaths of the human nervous system

Immunocytochemical method to identify myelin basic protein in oligodendroglia and myelin sheaths of the human nervous system

10.1002/ana.410070211

Crossref journal-article

Wiley

Annals of Neurology (311)

Abstract

AbstractTo study the distribution of basic protein (BP) and other myelin constituents immunocytochemically in human nervous tissue, we modified the unlabeled antibody enzyme (peroxidase‐antiperosidase) method. The technique is described here. Because the availability of unfixed tissue from human central nervous system is limited, we tested the method on blocks that had been fixed in formalin and embedded in paraffin, fixed and stored in 4% formalin, or frozen at autopsy and stored. We obtained the best results with paraffin blocks. BP antiserum scained oligodendroglia and myelin sheaths in the developing human nervous system. In the adult, myelin sheaths were well stained. Also, abnormalities associated with myelin breakdown could be identified in multiple sclerosis lesions. The results suggest that this method will be useful in studying the cellular distribution of myelin components in human demyelinating diseases.

Bibliography

Itoyama, Y., Sternberger, N. H., Kies, M. W., Cohen, S. R., Richardson, E. P., & Webster, H. DeF. (1980). Immunocytochemical method to identify myelin basic protein in oligodendroglia and myelin sheaths of the human nervous system. Annals of Neurology, 7(2), 157â166. Portico.

Authors 6

Yasuto Itoyama (first)
Nancy H. Sternberger (additional)
Marian W. Kies (additional)
Steven R. Cohen (additional)
Edward P. Richardson (additional)
Henry DeF. Webster (additional)

References 25 Referenced 69

AlvordECJr ShawCM HrubyS et al:Neuro‐allergic reactions in primates in Shiraki H Yonezawa T Kuroiwa Y (eds): The Aetiology and Pathogenesis of the Demyelinating Diseases. Proceedings of a Symposium Held in September 1973. Tokyo Japan Science Press 1976 pp203–216
10.1111/j.1471-4159.1975.tb06952.x
10.1056/NEJM197612232952604
10.1111/j.1471-4159.1975.tb04329.x
10.1080/00327487208061467
10.1111/j.1471-4159.1972.tb01382.x
10.1111/j.1365-2990.1976.tb00500.x
10.1111/j.1471-4159.1975.tb03849.x
10.1126/science.144.3624.1344
{'key': 'e_1_2_1_11_2', 'first-page': '216', 'article-title': 'Successful immunocytochemistry localization of myelin components in paraffin sections of human nervous tissue with preliminary observations on multiple sclerosis and metachromatic leukódystrophy lesions', 'volume': '103', 'author': 'Itoyama Y', 'year': '1979', 'journal-title': 'Trans Am Neurol Assoc'} / Trans Am Neurol Assoc / Successful immunocytochemistry localization of myelin components in paraffin sections of human nervous tissue with preliminary observations on multiple sclerosis and metachromatic leukódystrophy lesions by Itoyama Y (1979)
10.1111/j.1749-6632.1965.tb20199.x
MasonDY TaylorCR: The distribution of muramidase (lysozyme) in human tissues.J Clin Pathol124–132 1975 (10.1136/jcp.28.2.124)
10.1083/jcb.76.2.502
{'key': 'e_1_2_1_15_2', 'volume-title': 'The Neurons and Supporting Cells', 'author': 'Peters A', 'year': '1976'} / The Neurons and Supporting Cells by Peters A (1976)
10.1016/0006-8993(79)91068-0
10.4049/jimmunol.92.3.452 / J Immunol / Immunofluorescent localization of encephalitogenic protein in myelin by Rauch HC (1964)
10.4049/jimmunol.114.2_Part_1.630 / J Immunol / Neural antigens and induction of myelination inhibition factor by Seil FJ (1975)
{'key': 'e_1_2_1_19_2', 'first-page': '134', 'article-title': 'The use of thyroglobulin to induce antigenicity to small molecules', 'volume': '80', 'author': 'Skowsky WR', 'year': '1972', 'journal-title': 'J Lab Clin Med'} / J Lab Clin Med / The use of thyroglobulin to induce antigenicity to small molecules by Skowsky WR (1972)
{'key': 'e_1_2_1_20_2', 'volume-title': 'Immunocytochemistry', 'author': 'Sternberger LA', 'year': '1979'} / Immunocytochemistry by Sternberger LA (1979)
10.1177/18.5.315
10.1007/BF01217922
10.1073/pnas.75.5.2521
10.1073/pnas.76.3.1510
10.1136/jcp.27.1.14
10.1002/ana.410030403

Dates

Type	When
Created	20 years, 8 months ago (Jan. 1, 2005, 11:57 a.m.)
Deposited	1 year, 10 months ago (Nov. 2, 2023, 4:21 p.m.)
Indexed	2 days, 1 hour ago (Sept. 4, 2025, 9:47 a.m.)
Issued	45 years, 7 months ago (Feb. 1, 1980)
Published	45 years, 7 months ago (Feb. 1, 1980)
Published Online	20 years, 10 months ago (Oct. 7, 2004)
Published Print	45 years, 7 months ago (Feb. 1, 1980)

Funders 0

None

BibTeX

@article{Itoyama_1980, title={Immunocytochemical method to identify myelin basic protein in oligodendroglia and myelin sheaths of the human nervous system}, volume={7}, ISSN={1531-8249}, url={http://dx.doi.org/10.1002/ana.410070211}, DOI={10.1002/ana.410070211}, number={2}, journal={Annals of Neurology}, publisher={Wiley}, author={Itoyama, Yasuto and Sternberger, Nancy H. and Kies, Marian W. and Cohen, Steven R. and Richardson, Edward P. and Webster, Henry DeF.}, year={1980}, month=feb, pages={157–166} }

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