DOI: 10.1002/aja.1002020211. Experimental analysis of <i>Msx‐1</i> and <i>Msx‐2</i> gene expression during chick mandibular morphogenesis

Experimental analysis of Msx‐1 and Msx‐2 gene expression during chick mandibular morphogenesis

10.1002/aja.1002020211

Crossref journal-article

Wiley

Developmental Dynamics (311)

Abstract

AbstractHomeobox‐containing genes are thought to be involved in regulating pattern formation in a variety of tissues during embryogenesis. We have examined the expression of the homeobox‐related genes Msx‐1 and Msx‐2 during the development of the chick mandibular arch. Northern blot hybridization indicates that transcripts for both Msx‐1 (1.6 Kb) and Msx‐2 (3 Kb) are present in the mandibular arch as early as stage 18. The levels of both transcripts in the whole mandible decrease as cartilage is formed in vivo and in vitro. Using in situ hybridization, transcripts of Msx‐1 were localized in high amounts to the mesenchyme of the mesial tips of the arches. Msx‐2 transcripts were localized in high amounts to medial regions of the arches. Little or no hybridization of either probe was detected in the chondrogenic and myogenic regions of the arches. Transcripts of both genes were also excluded from calcified bone and cartilage. Our results further demonstrate that the mesial tip mesenchyme expressing Msx‐1 includes areas of highly proliferative cells and has in vitro chondrogenic potential. The region of mesenchymal cells expressing the Msx‐2 gene overlap with areas of developmentally programmed cell death which also contain very few proliferative cells and lack chondrogenic potential in vitro. These results are consistent with the possibility that Msx‐1 may be involved in the outgrowth of the mandibular arch and Msx‐2 may be involved in both developmentally programmed cell death and delineating the non‐chondrogenic region of the medial part of the mandibular arch. © 1995 Wiley‐Liss, Inc.

Bibliography

Mina, M., Gluhak, J., Upholt, W. B., Kollar, E. J., & Rogers, B. (1995). Experimental analysis of Msxâ1 and Msxâ2 gene expression during chick mandibular morphogenesis. Developmental Dynamics, 202(2), 195â214. Portico.

Authors 5

Mina Mina (first)
Jelica Gluhak (additional)
William B. Upholt (additional)
Edward J. Kollar (additional)
Barbara Rogers (additional)

References 56 Referenced 68

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Dates

Type	When
Created	20 years, 6 months ago (Feb. 26, 2005, 9:04 p.m.)
Deposited	1 year, 10 months ago (Oct. 26, 2023, 1:43 p.m.)
Indexed	3 months, 2 weeks ago (May 19, 2025, 8:30 a.m.)
Issued	30 years, 7 months ago (Feb. 1, 1995)
Published	30 years, 7 months ago (Feb. 1, 1995)
Published Online	20 years, 7 months ago (Feb. 3, 2005)
Published Print	30 years, 7 months ago (Feb. 1, 1995)

Funders 0

None

BibTeX

@article{Mina_1995, title={Experimental analysis of Msx‐1 and Msx‐2 gene expression during chick mandibular morphogenesis}, volume={202}, ISSN={1097-0177}, url={http://dx.doi.org/10.1002/aja.1002020211}, DOI={10.1002/aja.1002020211}, number={2}, journal={Developmental Dynamics}, publisher={Wiley}, author={Mina, Mina and Gluhak, Jelica and Upholt, William B. and Kollar, Edward J. and Rogers, Barbara}, year={1995}, month=feb, pages={195–214} }

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  "abstract": "<jats:title>Abstract</jats:title><jats:p>Homeobox\u2010containing genes are thought to be involved in regulating pattern formation in a variety of tissues during embryogenesis. We have examined the expression of the homeobox\u2010related genes <jats:italic>Msx</jats:italic>\u2010<jats:italic>1</jats:italic> and <jats:italic>Msx</jats:italic>\u2010<jats:italic>2</jats:italic> during the development of the chick mandibular arch. Northern blot hybridization indicates that transcripts for both <jats:italic>Msx</jats:italic>\u2010<jats:italic>1</jats:italic> (1.6 Kb) and <jats:italic>Msx</jats:italic>\u2010<jats:italic>2</jats:italic> (3 Kb) are present in the mandibular arch as early as stage 18. The levels of both transcripts in the whole mandible decrease as cartilage is formed in vivo and in vitro. Using in situ hybridization, transcripts of <jats:italic>Msx</jats:italic>\u2010<jats:italic>1</jats:italic> were localized in high amounts to the mesenchyme of the mesial tips of the arches. <jats:italic>Msx</jats:italic>\u2010<jats:italic>2</jats:italic> transcripts were localized in high amounts to medial regions of the arches. Little or no hybridization of either probe was detected in the chondrogenic and myogenic regions of the arches. Transcripts of both genes were also excluded from calcified bone and cartilage. Our results further demonstrate that the mesial tip mesenchyme expressing <jats:italic>Msx</jats:italic>\u2010<jats:italic>1</jats:italic> includes areas of highly proliferative cells and has in vitro chondrogenic potential. The region of mesenchymal cells expressing the <jats:italic>Msx</jats:italic>\u2010<jats:italic>2</jats:italic> gene overlap with areas of developmentally programmed cell death which also contain very few proliferative cells and lack chondrogenic potential in vitro. These results are consistent with the possibility that <jats:italic>Msx</jats:italic>\u2010<jats:italic>1</jats:italic> may be involved in the outgrowth of the mandibular arch and <jats:italic>Msx</jats:italic>\u2010<jats:italic>2</jats:italic> may be involved in both developmentally programmed cell death and delineating the non\u2010chondrogenic region of the medial part of the mandibular arch. \u00a9 1995 Wiley\u2010Liss, Inc.</jats:p>",
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