DOI: 10.1002/aja.1001400103. Three‐dimensional structure of the osmium‐impregnated golgi apparatus as seen in the high voltage electron microscope

Three‐dimensional structure of the osmium‐impregnated golgi apparatus as seen in the high voltage electron microscope

10.1002/aja.1001400103

Crossref journal-article

Wiley

American Journal of Anatomy (311)

Abstract

AbstractOsmication in an unbuffered aqueous solution of osmium tetroxide allows the forming face of the Golgi apparatus to be labeled in many cell types. This property was utilized to study the spatial configuration of this organelle by examining stereopairs of the same field taken at 1,000 KV after tilting a thick (1 to 7 μUm) section of − 7° or + 7° from the original (0°) position. When examined in 1 μm thick sections at magnifications ranging from 13,000 to 18,000 times, the osmic acid‐impregnated element of the Golgi apparatus of ganglion nerve cells, Leydig cells or Sertoli cells takes the appearance of a single layered polygonal network of tubules. This network can only be seen at electron microscope magnifications and is referred to as the primary network or structure of the forming face of the Golgi apparatus. When 2 to 7 μm thick sections are examined under progressively lower magnifications, the details of the primary structure remain discernible but become less conspicuous. The osmiophilic portion of the Golgi apparatus now extends over large areas of the cytoplasm to form an extensive continuous structure. This structure which is in the range of visibility of the light microscope is referred to as the secondary network or structure of the forming face. In ganglion nerve cells, the secondary structure consists of a perinuclear network showing slender projections reaching the nucleus and wider expansions approaching the cell surface; in the Leydig cells it appears as an ovoid structure located at one pole of the nucleus whereas in Sertoli cells it forms a cylindrical structure located in the main shaft of the cytoplasm and extending from the nucleus towards the lumen of the seminiferous tubule. Thus the forming face of the Golgi apparatus displays a primary structure; the tubular roughly polygonal network, which is similar in the three cell types and a secondary structure which varies from cell to cell.

Bibliography

Rambourg, A., Clermont, Y., & Marraud, A. (1974). Threeâdimensional structure of the osmiumâimpregnated golgi apparatus as seen in the high voltage electron microscope. American Journal of Anatomy, 140(1), 27â45. Portico.

Authors 3

A. Rambourg (first)
Y. Clermont (additional)
A. Marraud (additional)

References 28 Referenced 91

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{'key': 'e_1_2_1_4_1', 'series-title': 'C. R. Acad. Sci Paris, Série D.', 'first-page': '876', 'volume-title': "Observation, en microscopie électronique à haute tension, de l'appareil de Golgi sur coupes de 0.5 à 5 μ d'epaisseur", 'author': 'Carasso N.', 'year': '1971'} / Observation, en microscopie électronique à haute tension, de l'appareil de Golgi sur coupes de 0.5 à 5 μ d'epaisseur / C. R. Acad. Sci Paris, Série D. by Carasso N. (1971)
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{'key': 'e_1_2_1_9_1', 'first-page': '60', 'article-title': 'Sur la structure des cellules nerveuses', 'volume': '30', 'author': 'Golgi C.', 'year': '1898', 'journal-title': 'Arch. Ital. Biol.'} / Arch. Ital. Biol. / Sur la structure des cellules nerveuses by Golgi C. (1898)
{'key': 'e_1_2_1_10_1', 'first-page': '278', 'article-title': 'Sur la structure des cellules nerveuses des ganglions spinaux', 'volume': '30', 'author': 'Golgi C.', 'year': '1898', 'journal-title': 'Arch. Ital. Biol.'} / Arch. Ital. Biol. / Sur la structure des cellules nerveuses des ganglions spinaux by Golgi C. (1898)
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{'key': 'e_1_2_1_12_1', 'first-page': '1278', 'article-title': "Ultrastructure, polarité et reproduction de l'appareil de Golgi", 'volume': '245', 'author': 'Grassé P. D.', 'year': '1957', 'journal-title': 'C. R. Acad. Sci. Paris'} / C. R. Acad. Sci. Paris / Ultrastructure, polarité et reproduction de l'appareil de Golgi by Grassé P. D. (1957)
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{'key': 'e_1_2_1_20_1', 'first-page': '135', 'article-title': 'Mise en evidence par cytochimie ultrastructurale de compartiments physiologiquement differents dans un měme saccule Golgien', 'volume': '15', 'author': 'Ovtracht L.', 'year': '1972', 'journal-title': 'J. Microscopie (Paris)'} / J. Microscopie (Paris) / Mise en evidence par cytochimie ultrastructurale de compartiments physiologiquement differents dans un měme saccule Golgien by Ovtracht L. (1972)
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{'key': 'e_1_2_1_23_1', 'series-title': 'C. R. Acad. Sci., Paris, Série D', 'first-page': '981', 'volume-title': "L'appareil de Golgi: Examen en microscopie électronique de coupes épaisses (0.5–1 μ) après imprégnation des tissus par le tetroxyde d'osmium", 'author': 'Rambourg A.', 'year': '1970'} / L'appareil de Golgi: Examen en microscopie électronique de coupes épaisses (0.5–1 μ) après imprégnation des tissus par le tetroxyde d'osmium / C. R. Acad. Sci., Paris, Série D by Rambourg A. (1970)
{'key': 'e_1_2_1_24_1', 'first-page': '83A', 'article-title': "Structure tridimensionelle de la face de formation de l'appareil de Golgi examinée en microscopie électronique à très haute tension (1,000 KV) après imprégnation osmique des petites cellules nerveuses du ganglion de Gasser", 'volume': '14', 'author': 'Rambourg A.', 'year': '1972', 'journal-title': 'J. Microscopie (Paris)'} / J. Microscopie (Paris) / Structure tridimensionelle de la face de formation de l'appareil de Golgi examinée en microscopie électronique à très haute tension (1,000 KV) après imprégnation osmique des petites cellules nerveuses du ganglion de Gasser by Rambourg A. (1972)
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10.1016/S0022-5320(70)90167-X
{'key': 'e_1_2_1_28_1', 'first-page': '689', 'article-title': "Rôle de l'appareil de Golgi dans la synthèse des mucopolysaccharides. Etude cytochimique. I — Mise en évidence de mucopolysaccharides dans les vésicules de transition entre l'ergastoplasme et l'appareil. de Golgi", 'volume': '8', 'author': 'Thiéry J. P.', 'year': '1969', 'journal-title': 'J. Microscopie'} / J. Microscopie / Rôle de l'appareil de Golgi dans la synthèse des mucopolysaccharides. Etude cytochimique. I — Mise en évidence de mucopolysaccharides dans les vésicules de transition entre l'ergastoplasme et l'appareil. de Golgi by Thiéry J. P. (1969)
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Dates

Type	When
Created	20 years, 5 months ago (Feb. 26, 2005, 9:12 a.m.)
Deposited	1 year, 9 months ago (Nov. 1, 2023, 12:09 p.m.)
Indexed	1 year, 9 months ago (Nov. 12, 2023, 8:51 a.m.)
Issued	51 years, 3 months ago (May 1, 1974)
Published	51 years, 3 months ago (May 1, 1974)
Published Online	20 years, 6 months ago (Feb. 3, 2005)
Published Print	51 years, 3 months ago (May 1, 1974)

Funders 0

None

BibTeX

@article{Rambourg_1974, title={Three‐dimensional structure of the osmium‐impregnated golgi apparatus as seen in the high voltage electron microscope}, volume={140}, ISSN={1553-0795}, url={http://dx.doi.org/10.1002/aja.1001400103}, DOI={10.1002/aja.1001400103}, number={1}, journal={American Journal of Anatomy}, publisher={Wiley}, author={Rambourg, A. and Clermont, Y. and Marraud, A.}, year={1974}, month=may, pages={27–45} }

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