DOI: 10.1002/9780471729259.mc11a01s00. Cultivation and Laboratory Maintenance of <i>Chlamydia trachomatis</i>

Cultivation and Laboratory Maintenance of Chlamydia trachomatis

10.1002/9780471729259.mc11a01s00

Crossref journal-article

Wiley

Current Protocols in Microbiology (311)

Abstract

AbstractChlamydia species are obligate intracellular bacteria that require growth inside mammalian cells for propagation and survival. As a result, Chlamydia cannot be grown on conventional bacteriological medium. This property makes Chlamydia difficult organisms to grow and maintain in the laboratory. Up until 1965, passage in the yolk sac of the embryonated hen egg was the only way to isolate and propagate the organism. Since then, a tissue culture system has been available that allows easier laboratory culture of the Chlamydia species. However, with the exception of the LGV serovars, most C. trachomatis strains do not readily infect tissue culture cells. Chemical or mechanical assistance is used to increase their infectivity. Today, large numbers of infectious organisms can be purified through Renografin density gradient centrifugation of infected cell lysates. The ability to propagate C. trachomatis in the laboratory has greatly increased the understanding of the pathogenesis of C. trachomatis organisms.

Bibliography

Scidmore, M. A. (2006). Cultivation and Laboratory Maintenance of Chlamydia trachomatis. Current Protocols in Microbiology, 00(1). Portico.

Authors 1

Marci A. Scidmore (first)

References 11 Referenced 82

10.1128/IAI.31.3.1161-1176.1981
10.1128/MMBR.55.1.143-190.1991
10.1128/9781555818203.ch6
10.1016/0076-6879(94)36028-6
{'key': 'e_1_2_12_2_5_1', 'first-page': '81', 'article-title': 'Trachoma virus in chick embryo', 'volume': '43', 'author': "T'ang F.F.", 'year': '1957', 'journal-title': 'Natl. Med. J. China'} / Natl. Med. J. China / Trachoma virus in chick embryo by T'ang F.F. (1957)
{'key': 'e_1_2_12_2_6_1', 'first-page': '115', 'article-title': 'Global data on blindness', 'volume': '73', 'author': 'Thylefors B.', 'year': '1995', 'journal-title': 'B. World Health Organ.'} / B. World Health Organ. / Global data on blindness by Thylefors B. (1995)
{'key': 'e_1_2_12_2_7_1', 'first-page': '193', 'article-title': 'Chlamydia trachomatis genital tract infections—United States', 'volume': '46', 'author': 'World Health Organization', 'year': '1997', 'journal-title': 'Morb. Mortal. Weekly Rep.'} / Morb. Mortal. Weekly Rep. / Chlamydia trachomatis genital tract infections—United States by World Health Organization (1997)
{'key': 'e_1_2_12_3_2_1', 'volume-title': 'Current Protocols in Cell Biology', 'author': 'Phelan M.C.', 'year': '1998'} / Current Protocols in Cell Biology by Phelan M.C. (1998)
Schachter J.andWyrick P.B.1994. Seeabove.
{'key': 'e_1_2_12_3_6_1', 'volume-title': 'Biosafety in Microbiological and Biomedical Laboratories', 'author': 'Richmond J.Y.', 'year': '1999'} / Biosafety in Microbiological and Biomedical Laboratories by Richmond J.Y. (1999)
http://www.chlamydiae.com

Dates

Type	When
Created	19 years, 6 months ago (Jan. 31, 2006, 10:16 a.m.)
Deposited	2 years ago (Aug. 25, 2023, 12:54 p.m.)
Indexed	6 days, 15 hours ago (Aug. 23, 2025, 9:54 p.m.)
Issued	19 years, 6 months ago (Feb. 1, 2006)
Published	19 years, 6 months ago (Feb. 1, 2006)
Published Online	19 years, 1 month ago (July 1, 2006)
Published Print	19 years, 6 months ago (Feb. 1, 2006)

Funders 0

None

BibTeX

@article{Scidmore_2006, title={Cultivation and Laboratory Maintenance of Chlamydia trachomatis}, volume={00}, ISSN={1934-8533}, url={http://dx.doi.org/10.1002/9780471729259.mc11a01s00}, DOI={10.1002/9780471729259.mc11a01s00}, number={1}, journal={Current Protocols in Microbiology}, publisher={Wiley}, author={Scidmore, Marci A.}, year={2006}, month=feb }

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